On the significance of the plasmal reaction.
نویسنده
چکیده
THE 'plasmal reaction' has been widely used in cytology, and is generally interpreted as revealing the presence of aldehydes, but there is considerable doubt as to their source. According to Feulgen and his collaborators, who first described the reaction, the aldehydes (plasmal) are formed by decomposition of acetalphosphatides (plasmalogen) under the catalytic influence of mercuric chloride. According to Verne, they are formed by oxidation at unsaturated linkages in various forms of lipoid, the explanation of the action of mercuric chloride being either that it 'unmasks' the lipoids (Gerard) or that it destroys the inhibiting effect produced by formaldehyde when that substance is used in fixation. The techniques used for demonstrating the plasmal reaction vary considerably, having in common only the use of Schiff's reagent and the production of results in the cytoplasm and its inclusions, not in the nucleus. This paper presents evidence to show that the variations in technique are of importance and affect the results. There is no evidence that aldehydes are concerned in the reaction as practised by cytologists, but there is good evidence that oxides and hydroperoxides are the groups responsible in many cases, and that although acetal linkages will give rise to aldehydes under the conditions of the improved technique suggested, there is no histochemical evidence that such linkages are in fact present in the tissues investigated. Such substances have been prepared from tissues by Feulgen and his school, but the plasmal technique as usually practised does not show them clearly.
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ورودعنوان ژورنال:
- The Quarterly journal of microscopical science
دوره 90 1 شماره
صفحات -
تاریخ انتشار 1949